Interactive UV-Vis spectrophotometer simulator with animated optical bench, Beer-Lambert law calculations, mixture deconvolution, stray light effects, and calibration curve generation. Adjust wavelength, concentration, path length, molar absorptivity, noise, and stray light in real-time. Free. This simulated lab activity was developed by Dr. María Emilia Villanueva and collaborators from the Department of Basic Sciences at Universidad Nacional de Luján. article The material is designed for the undergraduate instrumental. Calibration: First of all, fill a cuvette with 3 mL water, take it into the spectrophotometer, set the wavelength to 405 nm and click on the “A=0” button. Take the cuvette out and pour it off. Experiment design: Using para -nitrophenol (pNF) and water, prepare 3 samples in separate cuvettes. Sim4T lets your students work their way through carefully designed experiments using a UV-Vis absorption spectrometer simulator.
[PDF Version]